Thiol-Oligo-Modification

Thiol Modifications

Thiol (SH) group can be linked to the 5' or 3' terminus of an oligonucleotide. This modification is used for oligo activation prior to linking to various types of molecules or support. The thiol groups can be used to attach a variety of products to the oligonucleotide. Common thiol-reactive groups include maleimides, halogens, iodoacetamides and pyridyldisulfides. This DNA modification is commonly used for the attachment of enzymes such as horseradish peroxidase or alkaline phosphatase.

Thiol C3 S-S	Desalted Qty.	5'-Mod	Internal	3' -mod
250 nmol	15 OD	NA	NA	$110
1.0 µmol	35 OD	NA	NA	$160
Dithiol (DTPA)	Desalted Qty.	5'-Mod	Internal	3' -mod
250 nmol	15 OD	$220	$220	NA
1.0 µmol	35 OD	$330	$330	NA
Thiol C6	Desalted Qty.	5'-Mod	Internal	3' -mod
250 nmol	15 OD	$80	NA	NA
1.0 µmol	35 OD	$110	NA	NA
Thiol C6 S-S	Desalted Qty.	5'-Mod	Internal	3' -mod
250 nmol	15 OD	$170	NA	NA
1.0 µmol	35 OD	$200	NA	NA

Purification

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD₂₆₀ In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

For additional information, please Contact us.