Bio-Synthesis offers Primers, Probes and Standards to Researchers around the World to help combat the new Coronavirus SARS-CoV-2 (COVID19) and to speed up accurate diagnosis of the virus.
Available primer and probes
ID
Label 5’
Oligonucleotide Sequence (5’>3’)
Label 3’
CDC US
Nucleoprotein – N Protein
2019-nCoV_N1-F
GACCCCAAAATCAGCGAAAT
2019-nCoV_N1-R
TCTGGTTACTGCCAGTTGAATCTG
2019-nCoV_N1-P
FAM
ACCCCGCATTACGTTTGGTGGACC
BHQ1
2019-nCoV_N2-F
TTACAAACATTGGCCGCAAA
2019-nCoV_N2-R
GCGCGACATTCCGAAGAA
2019-nCoV_N2-P
ACAATTTGCCCCCAGCGCTTCAG
2019-nCoV_N3-F
GGGAGCCTTGAATACACCAAAA
2019-nCoV_N3-R
TGTAGCACGATTGCAGCATTG
2019-nCoV_N3-P
AYCACATTGGCACCCGCAATCCTG
HKU Med
HKU-NF
TAATCAGACAAGGAACTGATTA
HKU-NR
CGAAGGTGTGACTTCCATG
HKU-NP
GCAAATTGTGCAATTTGCGG
TAMRA
CDC China
Nucleoprotein - N Protein
2019-nCoV-NFP
GGGGAACTTCTCCTGCTAGAAT
2019-nCoV-NRP
CAGACATTTTGCTCTCAAGCTG
2019-nCoV-NP
TTGCTGCTGCTTGACAGATT
ORF1ab
2019-nCoV-OFP
CCCTGTGGGTTTTACACTTAA
2019-nCoV-ORP
ACGATTGTGCATCAGCTGA
2019-nCoV-OP
CCGTCTGCGGTATGTGGAAAGGTTATGG
BBQ1
.
ORF1b-nsp14
HKU-ORF1b-nsp14F
TGGGGYTTTACRGGTAACCT
HKU- ORF1b-nsp14R
AACRCGCTTAACAAAGCACTC
HKU-ORF1b-nsp141P
TAGTTGTGATGCWATCATGACTAG
CDC
RNAse Protein
RP-F
AGATTTGGACCTGCGAGCG
RP-R
GAGCGGCTGTCTCCACAAGT
RP-P RNAse P
TTCTGACCTGAAGGCTCTGCGCG
BHQ-1
Others
References
CDC - US Centers for Disease Control and Prevention. (2020, February 4). Real-time RT-PCR panel for detection 2019-novel coronavirus.
Charite Virology - Corman et al. (2020, January 17). Diagnostic detection of 2019-nCoV by real-time RT-PCR.
CDC China - National Institute For Viral Disease Control and Prevention. (2020, January 21). Specific primers and probes for detection 2019 novel coronavirus.
HKU Med Protocol
World Health Organization (WHO)
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