Amino Linker Oligonucleotide Modification

Primary alkyamino modified oligonucleotides are commonly used in oligonucleotide labeling and conjugations towards such electrophilic moieties as active esters, halogenated aliphatic and aromatic derivatives¹.  

In general, labeling oligonucleotides with different reporter molecules requires a post-synthetic solution phase process, where both amino labeled oligonucleotide and the corresponding reporter NHS ester are dissolved in an aqueous organic solvent system. It is important that both reacting synthons are completely soluble in the reaction mixture.  Another important requirement for the reaction to be successful is that the reaction mixture is basic enough to drive the coupling reaction to completion and that the active ester is stable at least for the duration of the reaction.

3’-Amino modified oligonucleotides demonstrated resistance to 3’-exonuclease activity² and are also used as efficient polymerase chain terminator or a 3’-blocking group.

Bio-synthesis offers not only a wide variety of 3’-, 5’- and internally amino modified oligonucleotides but also their conjugates with peptides, proteins and antibodies¹.

 Reference:

1. http://www.biosyn.com/Bioconjugation.aspx

2. Zendegui, J. G.; Vasquez, K. M.; Tinsley, J. H.; Kessler, D. J.; Hogan, M. E., Nucleic Acids Res., 1992, 20, 307.