Bio-Synthesis offers Spacer 9 PEG oligonucleotide modification at various
scales. PEG Spacer 9 is a triethylene glycol chain 9 atoms long (6 carbons
and 3 oxygens). Spacer 9 can be incorporated either at the 5'-end or 3'-end of an oligo
or internally. Multiple insertions can be used to create long spacer arms.
Spacer 9 has been used for a number of different applications. It can form a non-nucleotide
bridges in hairpin loops of an oligonucleotides,1 link oligonucleotides
to epitopes for drug development,2 and for solid-phase immobilization
of hybridization probes.3 Multiple incorporations of Spacer 9 have been
used to form long, flexible linker arms between the two domains (double-helix forming
and triple-helix forming, respectively) of a bifunctional DNA oligonucleotide, in
order to maximize the binding flexibility of the two domains for their respective
targets.4 This Spacer 9 modified oligonucleotide have been used to form
a peptide nucleic acid PNA-DNA conjugate for use in site-directed
recombination applications,1,2 but can be used for any oligonucleotide-based
application requiring a long spacer arm.
Contact Bio-Synthesis for Spacer 9 PEG Oligonucleotide Modification
Service.
Purification:
Spacer modified oligonucleotide can be accomplished by various methods. Desalting or cartridge purification is acceptable, however, additional purification by HPLC is strongly recommended.
Price:
Price/modification. A 10% discount for more than two spacer modifications is applied.
| 5' Spacer 9 Modifications |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$40.00
$50.00
$75.00
$150.00
$300.00
$40.00
$50.00
$75.00
$150.00
$300.00
|
|
| Internal Spacer 9 Modifications |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$40.00
$50.00
$75.00
$150.00
$300.00
$40.00
$50.00
$75.00
$150.00
$300.00
|
|
| 3' Spacer 9 Modifications |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$65.00
$65.00
$100.00
$200.00
$400.00
$65.00
$65.00
$100.00
$200.00
$400.00
|
|
References:
-
Nelson, J.S., Giver, L., Ellington, A.D., Letsinger, R.L. Incorporation of Non-Nucleotide
Bridge into Hairpin Oligonucleotides Capable of High-Affinity Binding to the Rev
Protein of HIV-1. Biochemistry. (1996), 35: 5339-5344.
- Palma, E., Klapper, D.G., Cho, M.J. Antibodies as Drug Carriers III: Design of
Oligonucleotides with Enhanced Binding Affinity for Immunoglobulin G. Pharm. Res.
(2005), 22: 122-127.
- Beattie, W.G., Meng, L., Turner, S.L., Varma, R.S., Dao, D.D., Beattie, K.L.
Hybridization of DNA targets to glass-tethered oligonucleotide probes. Mol. Biotechnol.
(1995), 4: 213-225.
- Rogers, F.A., Vasquez, K.M., Egholm, M., Glazer, P.M. Site-directed recombination
via bifunctional PNA-DNA conjugates. Proc. Natl. Acad. Sci. USA (2002), 99:
16695-16700.