Bio-Synthesis offers Spacer 18 PEG oligonucleotide modification at various
scales. PEG spacer 18 is a hexaethylene glycol chain having 18 atoms long (12 carbons
and 6 oxygens). Spacer 18 can be incorporated at the 5'-end or 3'-end of an oligo
or internally. Multiple insertions can be used to create long spacer arms.
Spacer 18 has been used to form bold folds and hairpin loops in oligonucleotides1,2,
and for solid-phase immobilization of hybridization probes3. Spacer 18
has also been used to modify random primers used in whole genome amplification (WGA)-based
applications, as a way to eliminate self-priming events that form spurious DNA products
(that is, false-positive amplification) in the PCR reactions4.
Contact Bio-Synthesis for Spacer 18 PEG Oligonucleotide Modification
Service.
Purification:
Spacer modified oligonucleotide can be accomplished by various methods. Desalting
or cartridge purification is acceptable ,however, additional purification by HPLC
is strongly recommended.
Price:
Price/modification. 10% discount for more than two spacer modifications.
| 5' Spacer 18 Modifications |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$50.00
$60.00
$90.00
$180.00
$360.00
$50.00
$60.00
$90.00
$180.00
$360.00
|
|
| Internal Spacer 18 Modifications |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$50.00
$60.00
$90.00
$180.00
$360.00
$50.00
$60.00
$90.00
$180.00
$360.00
|
|
| 3'Spacer 18 Modifications |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$80.00
$80.00
$120.00
$240.00
$475.00
$80.00
$80.00
$120.00
$240.00
$475.00
|
|
References:
1. Salunkhe, M., Wu, T.F., Letsinger, R.L. Control of folding and binding of oligonucleotides
by use of non-nucleotide linker. J. Am. Chem. Soc. (1992), 114: 8768-8772.
2. Durand, M., Chevrie, K., Chassignol, M., Thuong, N.T., Maurizot, J. Circular
dichroism studies of an oligodeoxyribonucleotide containing a hairpin loop made
of a hexaethylene glycol chain: conformation and stability.Nucleic Acids Res. (1990),
18: 6353-6359.
3. Zhang, Y., Coyne, M.Y., Will, S.G., Levenson, C.H., Kawasaki, E.S. Single-base
mutational analysis of cancer and genetic diseases using membrane bound modified
oligonucleotides. Nucleic Acids Res. (1991), 19: 3929-3933.
4. Brukner, I., Paquin, B., Belouchi, M., Labuda, D., Krajinovic, M. Self-priming
arrest by modified random oligonucleotides facilitates the quality control of whole
genome amplification.Anal. Biochem. (2005), 339: 345-347.