Custom siRNA Synthesis - Road to gene silencing
Synthetic siRNA and shRNA constructs are two of the numerous options available. BSI provides the custom synthesis of sequence-specific siRNA for direct transfection, as well as the routine synthesis of shRNA oligos, ~60 bases long purified DNA oligos to be cloned to form shRNA constructs . Our siRNAs (small interfering RNAs) are double-stranded RNA molecules, made of 19 RNA nucleotides with two nucleotides (usually DNA bases) 3' overhangs. They are used to knockdown a specific gene of interest for identifying gene function, elucidating pathways and screening for potential new drug targets.
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Modifications and Labelings
Custom siRNA is available with a variety of fluorescent labels and other modifications. Alexa Fluor dyes provide superior performance and can be directly substituted for other common dyes. Other fluorescent dyes available include fluorescein, rhodamine, Cy3, and Cy5. Custom siRNA may be labeled at either the 3’ or the 5’ end of the sense and/or antisense strand, and labels do not affect the biological activity of the siRNA. As well as fluorescent labels, many other backbone and base modifications are available including amino linkers, thio linkers, biotin, dabcyl and phosphate modifications. View the full range of modification options Special modifications are also available on request; please contact our RNA oligo Technical Specialists.
All siRNA oligos are provided as a lyophilized pellet to ensure stability during delivery.
Synthesis Platform
Bio-Synthesis provides siRNA by using proprietary synthesis and purification processes with final products that are ready-to-use. There is no need for further time-consuming and expensive HPLC or PAGE purification. The high-throughput synthesis and purification processes enable fast turnaround times and high yields. Each duplex siRNA synthesis undergoes stringent quality control including MALDI-TOF mass spectrometry analysis that verifies identity of the component strands and analytical HPLC QC that verifies purity of the duplex itself, a level of QC unparalleled in the industry. Our high performance grade siRNA is economically priced allowing the use of highly pure siRNA in all routine RNAi experiments. For very sensitive applications, custom synthesis of HPLC-purified siRNA is also available upon request. Read more about siRNA synthesis platform and technology.
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