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Scorpions® Primers
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PNA Overview
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Labeled PNA
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Long Peptide Synthesis
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Catalog Peptides
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Regiospecific Modifications
Unnatural / Unusual Amino Acids
Peptide Labeling
Fluorescent Labeling
Enzyme Labeling
Isotope Labeling
Specialty Peptide Synthesis
Stapled Peptides
Cyclic Peptides
MAP Peptides
Additional Services
Peptide Conjugation
Cosmetic Peptides
Quality Control
Peptide Development Pipelines
Peptide Production Process
Design Tools
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Antibody Fluorescent Labeling
Antibody Bioconjugation
Antibody Characterization
Assay Development
Antibody Modifications
Antibody Modifications
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Secondary Detection Probes
Enzyme Labeling
Nanoparticle Labeling
Antibody Immobilization
Antibody Fragmentation
Antibody-Oligo Conjugation
Liposome Conjugation
Bioconjugation
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Oligo-Carrier Conjugation
Antibody Conjugation Services
Antibody Conjugation
Antibody-Enzyme Conjugation
Antibody Labeling
Antibody-polymer Conjugation
Antibody-Drug Conjugation
Antibody Agarose
Antibody Immobilization
F(ab')2 Preparation
F(ab')2-Enzyme Conjugation
Additional Conjugation Services
Bioconjugation Selection
Peptide bioconjugation
Protein bioconjugation
Enzyme bioconjugation
Reporter Molecule Labeling
Glycoconjugates
Drug Conjugation
PEGylation
Dextran Conjugation
Macrocyclics Metal Chelators
Dendritic bioconjugation
Gold Nanoparticles
Nano to Macro Particles
Small Molecule Bioconjugation
Diagnostic Conjugation
Biopharmaceutical Conjugation
Molecular Biology
Services Selected by Name
Molecular Biology Services
Antisense Oligonucleotides
DNA/RNA Synthesis
BNA Synthesis
Endotoxin Testing/Removal
Gene Synthesis
RNA Transcription Services
mRNA Gene Synthesis Services
lncRNA (miRNA, rRNA, shRNA)
Mutagenesis Services
PCR Product Cloning
Sub-cloning Services
siRNA and miRNA Services
Plasmid DNA Preparation
Services Selected by Application
Controls, standard and calibrators
Gene Silencing/Antisense
Functional Genomics/RNAi
Genotyping
In vivo Delivery
qPCR Probes
Cloning and Expression
Protein Expression
Molecular Biology Products
Catalog Products
BRAF Mutation Analysis Kits
Covid-19 Control and qPCR Probe Assay
Poly(A) carrier RNA
Additional Services
Protein Expression Overview
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E. Coli Protein Expression
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Quote Protein Expression Request Form
How To Get Your Quote:
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1-800-227-0627
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PROTEIN DETAILS
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Protein 1
A. Describe your protein
Protein Name:
*
Accession #:
Molecular Weight:
B. Provide your specification (please select ONLY ONE of three option below)
My DNA Template (ready for subcloning)
Construct (expression ready)
Synthesize from sequence below OR upload your file
or
Additional Notes
Add Item
Continue
PROTEIN EXPRESSION
<< Back
Expression System
Bacteria
Baculovirus/Insect
E.coli
Mammalian cells
Other:
Yeast
Cell Free System
Transient Transfection
HEK293
Other:
Stable Cell Line
CHO
HEK293
Other:
Has the gene been expressed in a host previously?
YES
NO
If yes, please provide us your protocol and experience in a separate file.
Expression Scale
mg
Or
liters
Protein Characteristics
Membrane Bound
Toxic to E. coli
Secreted
Protease
Enzyme
Nuclear Protein
Other (Please be specific):
Protein Applications
High-Throughput Screening
Structure Study
Activity Assay
Antigen
NMR
Other:
Protein Modification
Stable Isotope Labeling
15
N
13
C
PEGylation
Comments:
PROTEIN PURIFICATION
QC Requirements
Purity:
%
Endotoxin limit:
EU/mg
Concentration:
mg/ml
Standard Buffer
Other:
Tag Selection:
No
Yes, tag name:
Tag Removal:
No
Yes, cleavage enzyme:
Refolding:
No
Yes
Protein QC/Characterization:
SDS-PAGE
Native-PAGE
Coomassie Blue
Silver Staining
N-Terminal Sequence
Mass Spec
Western Blot
ELISA
Bioactivity Assay
HPLC
Endotoxin Test
Concentration Determination
Comments:
Purpose of Inquiry:
Quote
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Grant Application
Order Timeline:
Immediately
1 Month
3 Months
Not Sure
Submit
Note that starting synthesis scale is not the same as final yield.
Indicated starting scales are valid for unmodified Oligos of > 20 nt.
Indicate your minimum required yield in the "Sequence Notes" box.
Learn more about the difference between starting synthesis scale and oligo final yield