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Photo-regulation and photocleavage

Bio-Synthesis offers several light-triggered oligonucleotide modifications. These modified oligonucleotides are excellent tools for investigating the fundamental mechanisms in biological research. These light induced modified oligonucleotides allow for the temporal control of the base pairing process. In their caged form, the oligonucleotides are unable to participate in base pairing. It is only after exposure to UV light at 365 nm that the native oligonucleotides are released. Oligonucleotides containing NPOM-Caged-dT every five or six bases do not hybridize to their complementary strand. DEACM-dG is a convenient way to introduce a photocage at specific positions. These photoconvertible bases can be incorporated in the backbone, ribose, and nucleobase of an oligonucleotide to undergo various reversible or irreversible light-induced reactions.

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Our Advantages

  • Wide selection of modified RNA,DNA bases beyond 5-Mthyl C
  • Flexibility in oligo length and synthesis scales
  • Oligonucleotides are delivered as deprotected, desalted and/or purified
  • Quality checked by MALDI-TOF/LC-MS, analytical HPLC, PAGE
  • Specialize in producing newly-discovered and difficult -to-make modified bases
Product Modification Code 5 Prime Internal 3 Prime
PC Biotin [PC-Biotin] Y Y Y
DEACM Caged-dG [DEACM-dG] Y Y Y
PC Linker [PC-Linker] Y Y Y
PC Spacer [SpPC] Y Y Y
PC amino Modifier [PCAm] Y Y Y
Azobenzene [AzBen] Y Y Y
NPOM Caged-dT [NPOM-dT] Y Y Y
CNVK Photo Cross Linker [CNVK] Y Y Y

Quality Control: Analytical HPLC, Gel eletrophoresis, and  MALDI-TOF Mass Spectrometry

Delivery times: 5-7 Working days

Packaging:    Dried

Shipping conditions: Room temperature

Storage conditions: -20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

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Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please Call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."