Peptide Modifications

Bio-Synthesis offers hundreds of peptide modifications to meet your custom peptide needs. These peptide modifications can be used to create synthetic peptide with the exact conformation or characteristics needed for specific applications. Large numbers of modified amino acids are focused on post-translation modifications (PTM) that naturally occur in vivo, while others are pharmacologically modified or stable isotope labeled. Additionally, tags, proteins or oligonucleotides can be chemically conjugated to these peptides through Bio-Synthesis. With over 30 years of experience in providing modified peptide synthesis, we are your best choice for producing custom modified peptides on time and on budget. Our peptide modification services include but are not limited to the following:

• Fluorophores and Quenchers: Emitting and Quenching dyes for FRET and other dyes
• Attachment Chemistry: Linkers, Spacers and Polyethylene Glycol (PEG) modifications
• Unnatural Amino Acids: D-stereoisomers, Unnatural and special amino acids
• Post-translational Modifications (PTMs): Myristoylation, Palmitoylation, Glycosylation, Phosphorylation, Citrullination, Methylation, Succinylation
• Isotopically Labeled (heavy) Amino Acids: Carbon 13 (C13), Nitrogen 15 (N15) and Deuterium (H2)
• Immunogen Conjugation: Carrier protein conjugates, Lipids and Nucleic acids
• Specialty Peptides: Stapled peptides, Cyclization, Dimerization and many others
• Miscellaneous Modifications: Biotinylation, Carboxymethylation (CAM), Protecting group, Bioconjugation

Modified Peptide Synthesis Services

A selection of our peptide modification capabilities are listed below. This list is by no means complete. Please contact us if you require other peptide modifications.

• Amidation
• Peptide alcohols and aldehydes modification
• C-terminal esters and thioesters
• Multiple Antigen Peptides (MAP)

• PEGylation
• Acetylation, acylations (e.g. lipopeptides)
• C-terminal esters and thioesters
• Biotinylation
• Phosphorylation, sulfation and glycosylation
• Introduction of maleimido groups, chelating moieties, chromophores, and fluorophores

• Single or multiple disulfide bridges
• Head-to-tail cyclization
• Side chain cyclization (e.g. lactam bridge, thioether)
• Hydrocarbon-stapled peptides

• Hydroxyethylene isosteres, thioethers, reduced peptide bonds, retro-inverso peptides
• Depsipeptides
• Incorporation of unusual amino acids, D-enantiomers, α- and N-methylamino acids

• Heavy isotope labeling (15N, 13C)
• Conjugation to a carrier protein
• Conjugation to imaging agents
• FRET substrates with various fluorophore/quencher pairs
• Peptide-DNA conjugation
• Peptide-RNA conjugation
• Peptide-enzyme labeling

• Peptide agarose attachment
• Peptide nanoparticle conjugation
• Peptide gold conjugation
• Peptide membrane, slide and plate attachment