Symmetric Doubler Branch Oligo Synthesis
1,2 Symmetric dendrimers oligonucleotide modification offer serveral advantages: 1) Incorporation of label using g-32P-ATP and polynucleotide kinase increases in proportion to the number of 5'-ends. 2) Fluorescent signal also increases in proportion to the number of 5'-ends, if spacers are incorporated between the labels and the ends of the branches. 3) When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the 'bottom up' approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target.
Product Information
Symmetric Doubler Branch Oligo Synthesis
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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