Psoralen C2 Oligonucleotide Modification
Psoralens are a class of naturally occurring heterocyclic compounds which intercalates between bases in double-stranded or triple stranded DNA. Upon exposure to long wavelength light (350 nm) Psoralen forms covalent linkages to Thymidine (cyclobutane linkage). Psoralen is a bifunctional reagent and can form either a monoadduct, linking one adjacent thymidine on the same or complimentary strand, or a diadduct, linking adjacent thymidines on the same or complimentary strands. Diadducts formed between adjacent thymidines are photoreversable with short wavelength UV light (254 nm).
Psoralen C2 modified oligonculeotide is designed to crosslink to a T base adjacent to the 3'-terminus of the opposite strand of a double stranded DNA. Psoralen C6 is intended to fulfill the same purpose but, with the longer spacer, crosslinks to the triple strand of triplex DNA.Max excitation=330nm, observed at max 395nm.
Product Information
Psoralen C2 Oligonucleotide Modification
cross linked base, other label and conjugates
250.06 (100.0%), 251.06 (17.7%), 352.06 (2.9%)
C, 54.87; H, 4.32; O, 1.97; P, 8.84
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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