Oyster® 550 Fluorescent Dye Oligo Labeling
The Oyster®-550 fluorophore can be incorporated at any position within an oligonucleotide. Oyster-550 is a negatively charged cyanine based dye which is well suited for replacing Cy3, Alexa Fluor® 555, Dylight® 550 fluorophores. The Oyster®-550 fluorophore is especially recommended for the replacement of Cy3 in applications such as microarrays and FISH.
A primary amine modified oligonucleotide allows covalent attachment of the Oyster 550 fluorophore via NHS conjugation chemistry. However, other cross-linking chemistries are also available depending on particular project specifications. After oligonucleotide synthesis and dye conjugation, a dual HPLC purification is used to remove failure sequences and excessive dye quantitatively to obtain a full length labeled oligonucleotide.
Product Information
Oyster® 550 Fluorescent Dye Oligo Labeling
Extinction Coefficient, ε(λ):
150,000 M-1 x cm-1
Lyophilized; Protect from light
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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