O6-Phenyl-2'deoxyinosine Oligonucleotide Modification
O6-Phenyl-2' deoxyinosine, O6-Ph-dI, is a minor base modification. O6-phenyl-2'-deoxyInosine (O6-Ph-dI) is classified as convertible dA. After incorporation into an oligo, under mild ammonium hydroxide deprotection with a suitable diamine in a post synthetic substitution reaction. The reaction of O6-phenyl on the inosine base with a primary amine displaces the oxygen atom, and converts the nucleotide into a N6-substituted dA. O6-Ph-dI modified oligonucleotides have been used as affinity purification matrices when reacting with1,4-diaminobutane to form a DNA molecule with active amines at the N6 positions of several A positions in the oligo. Oligos containing O6-Phenyl-dI modifications are used in the studies that are requiring cross-linking at A position(s) between an oligo and an enzyme.
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O6-Phenyl-2'deoxyinosine Oligonucleotide Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
C.L. Larson and G.L. Verdine, Nucleic Acids Res., 1992, 20, 3525.
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