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The 5’-Dichloro-dimethoxy-fluorescein dye (JOE) is a traditional fluorophore highly useful for automated DNA sequencing.   For Applied Biosystems DNA PRISM sequencers, the dye sets FAM/JOE/TAMRA/ROX and FAM/JOE/NED/ROX can be employed. JOE’s emission falls between the other dyes used in automated sequencing, allowing it to be resolved from FAM and TAMRA. This allows multiplex detection without causing significant signal bleed-over into other channels.  It exhibits low sensitivity to pH as the fluorescein dye is less prone to quenching by protonation, accounting for the decrease in signal of mere 30% at pH 5.  Additional tributes are high fluorescent quantum yield (number of photons emitted per photon absorbed) of 0.581, high extinction coefficient (5,000 L/mol.cm), and stability under deprotection using ammonium hydroxide.  

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Product Information

 

Product Name:

JOE, 4-5-Dichloro carboxy fluorescein Oligonucleotide Labeling

Category:

Fluorescent dye Label, ATTO520 Alternative

Modification Code:

[JOE]

Chemical Formula:

C29H26Cl2NO11P

Exact Mass:

665.06

Formula Weight:

666.40

Absorption λmax:

520 nm

Emission (nm):

548 nm

Extinction Coefficient, ε(λ):

82,000

Structure:

Bio-Synthesis Inc. Oligo Structure

Purification:

HPLC or PAGE

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.