Janelia 525 Oligo Labeling
Janelia 525, a yellow fluorescent dye, is cell permieable with excitation maximum = 525 nm; emission maximum = 549 nm; quantum yield = 0.91; Extinction coefficient = 122,000 M-1cm-1; Correction factor A280 = 0.185. Janelia 525 is offered as an alternative to Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532
Janelia 525 modified oligonucleotide can be multiplexed for two color imaging with Janelia Fluor 635. It is suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells).
The Janelia 525 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failed sequences during first HPLC purification. After labeling, a second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Product Information
Janelia 525 Oligo Labeling
Extinction Coefficient, ε(λ):
122,000 M-1cm-1
Fluorescence QuantumYield, η:
0.91
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 14 987 PMID: 28869757
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