DyLight 405 Oligonucleotide Labeling
Bio-Synthesis offers DyLight™ 405 fluorescent dye oligonucleotide labeling services. DyLight™ 405 is a blue fluorescent dye that can be use to replace Alexa405, Cascade Blue and AMCA. This water soluble dye allows high oligo-dye conjugation ratio without causing precipitation.
DyLight™ 405 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification follow by additional step of ultrafiltration is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for DyLight 405 fluorescent dye oligonucleotide labeling services.
Product Information
DyLight 405 Oligonucleotide Labeling
Fluorescent dye label, Alexa405, Cascade Blue, AMCA Alternative
Extinction Coefficient, ε(λ):
30,000
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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