Digoxigenin Oligonucleotide Labeling
Bio-Synthesis provides digoxigenin oligonucleotide labeling services at 5', 3' and internally using NHS ester cross-linking chemistry that reacts with an amine group to form an amide. Digoxigenin, Digoxigenin-3-O-methylcarbonyl-epsilon-aminocaproic acid NHS ester, is a member of the steroid family found in Digitalis plants1. This is a hapten which can used to raise antibodies with considerably higher affinities for them than other antibodies do for their targets makes haptens particularly desirable as affinity tags for oligonucleotides2.
Digoxigenin, Dig labeld probes are commonly use in application such as, in situ hybridization, Northern and Southern blotting. After hybridization to their targets, these Dig-labeled probes are detected with anti-Dig antibodies that are labeled with dyes (for primary detection) or enzymes (for secondary detection using a fluorogenic, chemiluminogenic, or colorimetric substrate specific for the enzyme). For optimal signal, Bio-Synthesis recommends modifying the oligonucleotide probe with three Dig molecules, spaced about 10 bases apart. Starting scale for Dig-labeled oligonucleotide is 250 nmole, dual HPLC purification is highly recommended.
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Product Information
Digoxigenin Oligonucleotide Labeling
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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