DBCO-TEG Cyclooctyne Oligonucleotide Modification
Bio-Synthesis offer DBCO-TEG, a lonkger linker arm of DBCO modified oligonucleotide synthesis. DBCO-TEG is a dibenzocyclooctyl structure with 15 atom triethylene glycol spacer arm which is highly reactive towards azides without the need for copper catalysis and depending onthe azide been used, the reaction normally complete in 4-17 hours at room temperature. This modification can be place at any position within an oligonucleotide.
For 5' modification, we use 5'-DBCO TEG phosphoramidite chemistry in which the very hydrophobic DBCO moiety is separeated from the oligonucleotide with a PEG spacer. For internal DBCO oligo modification, a primary amine modified oligo is used to conjugate DBCO post-synthethically via NHS ester cross linking chemistry. Dual HPLC purification is requuire for this process.
Contact us for DBCO oligonucleotide modification services.
Product Information
DBCO-TEG Cyclooctyne Oligonucleotide Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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