DBCO-dT Oligo Modification
Bio-Synthesis offers serveral oligonucleotide modifications for copper-free click reaction. We offer several dibenzon-cyclooctyne (DBCO) modifiers, such as 5'-DBCL-TEG, a 15 atom triethylene glycol spacer. DBCO serinol may be inserted in between two bases at the phosphate backbone or DBCO-dT for inserting a DBCO group at any position within the oligonucleotide. DBCO modified oligos can be conjugated with other biomolecules containing azide functional group in DMSO, or aqueous buffers at room temperature. The reaction is robust with no side reactions, forming thermally and hydrolytically stable 1,2,3-triazole linkages.
Product Information
DBCO-dT Oligo Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Rostovtsev, V.V., et al., A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes. Angew Chem Int Ed Engl, 2002. 41(14): p. 2596-9.
- Moses, J.E. and A.D. Moorhouse, The growing applications of click chemistry. Chem Soc Rev, 2007. 36(8): p. 1249-62.
Modified oligo use for Click Chemistry are sold by Bio-Synthesis for the end-user's internal research purpose only. See
www.baseclick.org for further details.
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