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Bio-Synthesis offers Black Hole Quencher®-1 dye oligonucleotide modification. BHQ-1 can be place at 5' or 3' terminus or internally of an oligonucleotide. BHQ®-1 labeled oligonucleotides are used in many applications, including dual-labeled fluorogenic probes for real-time PCR. This dark quencher has strong absorption range of 480 nm - 580 nm, which provides excellent quenching of fluorophores that fluoresce in this range, such as FAM, TET, CAL Fluor® Gold 540, JOE, HEX, CAL Fluor Orange 560, and Quasar® 570 dyes. BHQ-1 dye may quench fluorescence by both FRET and static quenching mechanisms. Contact Bio-Synthesis for BHQ1 Quencher Oligonucleotide Synthesis and Modification Services.

Product Information

 

Product Name:

Black Hole Quencher 1 - BHQ1 Oligonucleotide Modification

Alternate Name:

Black Hole Quencher

Category:

Quencher

Modification Code:

[BHQ1]

Chemical Formula:

C24H25N6O8P

Exact Mass:

556.15

Formula Weight:

556.47

m/z:

556.16 (100%), 557.15 (26.6%), 558.15 (5.5%), 557.14 (2.2%)

Elemental Analysis:

C, 51.80; H, 4.53; N, 15.10; O, 23.00; P, 5.57

Absorption λmax:

534 nm

Structure:

Bio-Synthesis Inc. Oligo Structure

Purification:

HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.