Azide dT Oligo Modification
Azide dT can be introduced into any position within an oligonucleotide during oligonucleotide synthesis. The azide functionalized oligonucleotide allows attachment to biomolecules modified with an alkyne functional group using click chemistry. Conjugation of the azide-modified oligonucleotide to a terminal alkyne-modified oligonucleotide, peptide or other biomolecule occurs with extremely high region-selectivity and efficiency (1,2,3). A copper-free click reaction is especially desirable for in vivo application as well as for the production of circular oligonucleotides which has been shown to be very stable in a serum environment.
Please note: Bio-Synthesis does not offer Copper based Click Chemistry, we only offer Cooper free click chemistry.
For Copper based Click Chemistry please contact baseclick GMBH.
Product Information
Azide dT Oligo Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
- Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
- Kumar, R., El-Sagheer, A., Tumpane, J., Lincoln, P., Wilhelmsson, L.M., Brown, T. Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry. J. Am. Chem. Soc. (2007), 129: 6859-6864.
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