Azide C6 Oligo Modification
Azide C6 modified oligonucleotides are useful tools for copper-free click reagents. The azide C6 NHS ester allows the introduction of the azide C6 functional group at the 5’-end of an amino-modified oligonucleotide after synthesis. This azide functionalized oligonucleotide also allows the attachment of other biomolecules modified with an alkyne group using click chemistry and copper (I) iodide as a catalyst for the conjugation of the azide-modified oligonucleotides to a terminal or internally alkyne-modified oligonucleotide, peptide or other biomolecules with extremely high region-selectivity and efficiency (1,2,3). A copper-free reaction is especially desirable for in vivo application as well as for the production of circular oligonucleotides which has been shown to be very stable in the serum environment.
Product Information
Azide C6 Oligo Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
- Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
- Kumar, R., El-Sagheer, A., Tumpane, J., Lincoln, P., Wilhelmsson, L.M., Brown, T. Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry. J. Am. Chem. Soc. (2007), 129: 6859-6864.
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