Azide C3 Oligo Modification
Azide C3 modifications can be introduced at the 3'-end of oligonucleotides by a direct chemical synthesis method. Azide modified oligonucleotides can react with dibenzocyclooctyl (DBCO) modified biomolecule in organic solvents, such as DMSO, or also in aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. This azide functionalized oligo can be also be attached to alkyne modified biomolecules through a click reaction (1,2,3).
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Azide C3 Oligo Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
- Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
- Kumar, R., El-Sagheer, A., Tumpane, J., Lincoln, P., Wilhelmsson, L.M., Brown, T. Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry. J. Am. Chem. Soc. (2007), 129: 6859-6864.
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