Azide C2 NHS Oligo Modification
Azide C2 C6 linker modified oligonucleotides are azide tagged oligonucleotides useful for Click Chemistry. Click chemistry involves pairs of functional groups that rapidly and selectively react with each other. Click Chemistry is now widely used in the biosciences for coupling reactions since they occur under mild and in aqueous conditions. Azide C2 C6 linker modifiers are added post-synthetically to oligonucleotides containing a reactive primary amino group with the help of the azide C2 NHS ester. Introduction of this functional group into oligonucleotides can be done at the 5’- or 3’-end as well as at internal positions. As a result, the alkyne group is separated from the oligo by a spacer arm of varying length, which serves to reduce steric interaction between the reactive group and the oligonucleotide. Azide functionalized oligonucleotides can be attached to alkyne modified biomolecules using Click Chemistry and copper (I) iodide as a catalyst for the conjugation of azide-modified oligonucleotides to terminal alkyne-modified oligonucleotides, peptides or other biomolecules with extremely high region-selectivity and efficiency (1,2,3).
Product Information
Azide C2 NHS Oligo Modification
dual HPLC or dual HPLC/PAGE
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
- Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
- Kumar, R., El-Sagheer, A., Tumpane, J., Lincoln, P., Wilhelmsson, L.M., Brown, T. Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry. J. Am. Chem. Soc. (2007), 129: 6859-6864.
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