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Bio-Synthesis offers ATTO 700 fluorescent dye oligonucleotide labeling services. ATTO 700 has characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and has high thermal and photo-stability.

ATTO 700 is a zwitterionic dye with electrically neutral charge after coupling. ATTO 700 is a strong electron acceptor dye. Its fluorescence can be efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 670 - 715 nm.  They dye can be use as an alternative dye for Cy5.5,  IRD 700 fluorescent dye. 

  • Replacement dye for Cy5.5, IRD700
  • High fluorescence yield
  • Excellent thermal and photo-stability
  • Fluorescence quenching by guanine and tryptophan
  • Very Hydrophilic
  • Zwitterionic dye
ATTO700 wavelength
ATTO 700 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 700 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

ATTO 700 Fluorescent Dye Oligonucleotide Labeling

Category:

Fluorescent dye Label, Cy5.5, IRD700 Alternative

Absorption λmax:

700

Emission (nm):

719

Extinction Coefficient, ε(λ):

120,000

Fluorescence QuantumYield, η:

25

Fluorescence Lifetime, τ:

1.5

Purification:

dual HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.