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Bio-Synthesis offers ATTO 680  fluorescent dye oligonucleotide labeling services. ATTO-680  is a fluorescent labels for the red spectral region.  Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability.

ATTO 680 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 645 - 695 nm. A suitable excitation source for the dye is the Krypton-Ion laser using the 676 nm line or a diode-laser emitting at 670 nm. They dye can be use as an alternative dye for Cy5.5.

  • Replacement dye for Cy5.5
  • High fluorescence yield
  • Excellent thermal and photo-stability
  • Fluorescence quencing by quanine, tryptophan
  • Very hydrophilic
  • Zwitterionic dye
ATTO680 Wavelength
ATTO 680 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 680 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

ATTO 680 Fluorescent Dye Oligonucleotide Labeling

Category:

Fluorescent dye Label, Cy5.5 Alternative

Modification Code:

[ATTO680]

Excitation (nm):

680

Emission (nm):

695

Extinction Coefficient, ε(λ):

12,5000

Fluorescence QuantumYield, η:

30

Fluorescence Lifetime, τ:

1.8

Purification:

Dual HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.