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Bio-Synthesis offers ATTO 647  fluorescent dye oligonucleotide labeling services. ATTO 647  is a fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high photostability with good water solubility.

The fluorescence of ATTO 647 is excited most efficiently in the range 615- 660 nm, a suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.  

  • Replacement dye for Cy5, Alexa 647
  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Stable at pH 2-8
  • Zwitterionic dye
ATTO 647 Wavelength
ATTO 647 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 647 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

ATTO 647 Fluorescent Dye Oligonucleotide Labeling

Category:

Fluorescent dye Label, ATTO647

Absorption λmax:

645

Extinction Coefficient, ε(λ):

120,000

Fluorescence Maximum, λfl:

669

Fluorescence QuantumYield, η:

20

Fluorescence Lifetime, τ:

2.3

Purification:

dual HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.