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Bio-Synthesis offers ATTO 465 fluorescent dye oligonucleotide labeling services. ATTO 465 is a fluorescent label derived from the well-known dye Acriflavin. ATTO 465 is moderately hydrophilic. Its fluorescence can be excited efficiently in the range 420-465 nm. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. In aqueous solution the dye shows a surprisingly large Stokes-shift of 55 nm. The dye shows intense and long-lived phosphorescence in solid matrix, in particular at low temperatures.  This dye can be use to replace LC480 Cyan 500

  • Replacement dye for LC480, Cyan500
  • High fluorescence yield
  • Large Stoke-shift in aqueous solution
  • High triplet yield, intense phosphorescence in solid matrix
  • Hydrophilic
  • Cationic dye derive from well-known Acriflavine


ATTO 465 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 465 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

ATTO 465 Fluorescent Dye Oligonucleotide Labeling

Category:

Fluorescent dye Label, LC 480 Cyan 500 Alternative

Modification Code:

[ATTO465]

Absorption λmax:

453 nm

Emission (nm):

508 nm

Extinction Coefficient, ε(λ):

75,000

Fluorescence QuantumYield, η:

55

Fluorescence Lifetime, τ:

2.2

Structure:

Bio-Synthesis Inc. Oligo Structure

Purification:

dual HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.