Amino C6-U, a minor RNA base, can be incorporated at any position within an oligonucleotide during synthesis. This modification provides an active primary amino group that can be further used to conjugate with a variety of moieties such as biotin, digoxigenin, fluorescent dyes, quenchers, and magnetic beads or enzymes such as HRP or alkaline phosphatase. The primary amine labelled oligos can also be conjugated to carboxyl functional groups. Conjugation to carboxyl functional groups is typically for solid supports applications using EDC mediated reaction.
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Extinction Coefficient, ε(λ):
8.4
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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