Bio-Synthesis offers Alexa 568 fluorescent dye oligonucleotide labeling services. Alexa 568 is a orange fluorescent dye with excitation suitable for the 568 nm laser line on the Ar-Kr mixed-gas laser. This dye is pH-insensitive over a wide molar range which permit stable signals for imaging and flow cytometry application. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor® 568 is a substitute for Rhodamine Red, this molecules can be attached to oligo at multiple sites at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
Alexa 568 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 568 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.