Bio-Synthesis offers Alexa 488 fluorescent dye oligonucleotide labeling services. Alexa 488 is a green fluorescent dye with excitation suitable for the 488 nm laser line. This dye is pH-insensitive over a wide molar range which permit stable signals for sensitive detection in live and fixed cells. It is commonly used with Alexa Fluor® 350, 594, and 647 dyes for multiplexing. It is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM. It is also used extensively to label conjugates for SIM, STED, and two-photon excitation (TPE) microscopy. Alexa Fluor® 488 dye can be use to replace Cy2 or fluorescein (FITC), this dye can be attached to oligo at multiple sites at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
Alexa 488 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 488 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.