Bio-Synthesis offers Alexa 430 fluorescent dye oligonucleotide labeling services. The fluorescence conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emiision beyond 500 nm (exicitation and emission maixma ~4334/539 nm). This compound requires a primary amine for conjugation onto an oligonucleotide via NHS ester conjugation chemistry. Click reaction is also available.
Alexa 430 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 430 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.