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Bio-Synthesis offers Alexa 430 fluorescent dye oligonucleotide labeling services.  The fluorescence conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emiision beyond 500 nm (exicitation and emission maixma ~4334/539 nm).  This compound requires a primary amine for conjugation onto an oligonucleotide via NHS ester conjugation chemistry. Click reaction is also available.

  •  Large Stokes shift
  • Absorption at 434 nm
  • Emission at 539 nm

Alexa 430 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for Alexa 430 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

Alexa 430 Oligo Labeling

Category:

Fluorescent dye Label

Modification Code:

[Alexa430]

Chemical Formula:

C22H22F3NOS

Exact Mass:

485.11

Formula Weight:

485.47

Excitation (nm):

434 nm
            

Emission (nm):

539 nm
            

Extinction Coefficient, ε(λ):

16,000

Structure:

Bio-Synthesis Inc. Oligo Structure

Purification:

Dual HPLC required

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.