8-Bromo-2'-deoxyguanosine Oligonucleotide Modification
Photoreactive halogenated nucleoside such as 8-bromo-dG are often used in crystallography for DNA structure studies1.When incorporated into a DNA molecule, the multi-wavelength anomalous dispersion (MAD) technique can be applied to obtain the phase information necessary to correctly calculate the electron density for the unit cell of the molecule under study. Because the MAD technique allows for the measurement of all the diffraction data with the same sample, is a much simpler to use than the traditional multiple isomorphous replacement (MIR) method for phase determination, which requires the synthesis of, and collection of diffraction data from, multiple heavy-atom isomorphic derivatives of the original molecule2.
They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA, Protein-RNA, DNA-RNA complexes in crosslinking experiments. When incorporation of 5-Br-dC (and 5-Br-dG) into a 22-base dC-dG oligo, the oligo are readily to flip into the Z-DNA conformation in 10 mM MgCl2. This oligo was used as a probe to detect Z-DNA binding proteins3. 8-Bromo-dG can be place at any position within an oligonucleotide.
Product Information
8-Bromo-2'-deoxyguanosine Oligonucleotide Modification
Crystallography, protein cross-linking studies
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
1. Hendrickson, W.; Ogata, C. Phase determination from multiwavelength anomalous diffraction measurements. Meth. Enzymol.. (1997), 276: 494-523.
2. Walsh M.A.; Evans G.; Sanishvili R.; Dementieva I.; Joachimiak, A. MAD data collection - current trends. Acta Cryst. (1999), D55: 1726-1732.
3. Herbert, A.G.; Rich, A. A method to identify and characterize Z-DNA binding proteins using a linear oligodeoxynucleotide. Nucleic Acids Res. (1993), 21: 2669-2672.
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