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7-deaza-8-aza dG is a modified DNA base that has been used in analyzing DNA segment that are rich in G content. In DNA research, DNA base pairing are disrupted in G-rich segments becauseof their ability to create inter- and intra- strand hydrogen bonding. This aggregation causes enzymatic disruption so sequencing and PCR experiments become highly problematic. In probe experiments, these segments are not accessible due to this aggregation of G residues on the probe and/or target. The problem arises from extra hydrogen bonding forming at the N7 position of G1 .  The substitution of base G with 7-deaza-8-aza-dG (pyrazolo[3,4-d]pyrimidine) (PPG) for G were evaluated by Seela's group  where the N7 and C8 atom of the G base are flipped while allowing the modified base to retain the same electron density as the guanine ring system.

The 7-deaza-8-aza-G - C base pair was found to be stable relative to G - C by around 1° per insertion. This stability enhancement has led to interest in the use of PPG in diagnostic probe applications.

Related Products:

  • 7-Deaza-dA

Contact BioSynthesis for more information regarding 7-deaza-8-aza-dG DNA Modification

Product Information

 

Product Name:

7-deaza-8-aza-dG DNA Modification

Category:

2' Deoxyribonucleoside analogs

Modification Code:

[7-Deaza-8-Aza-dG]

Structure:

Bio-Synthesis Inc. Oligo Structure

Purification:

HPLC or PAGE

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.