7-Deaza-8-aza-dA DNA Modification
7-deaza-8-aza-dA, and 7-deaza-8-aza-A lack group that are critical for hydrogen bonding. 7-Deaza-8-aza-A and 7-deaza-8-aza-G (PPG) monomers are isomers of A and G and have similar electron density. Their presence in oligos is slightly stabilizing relative to A and G.
7-deaza modified base does not lead to aggregation in G-rich oligos and can be easily prepared and isolated. 5' fluorescein labeled oligos with 7-deaza-8-aza A modified base are much less quenched than the equivalent G oligos.
Product Information
7-Deaza-8-aza-dA DNA Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- F. Seela and H. Driller, Helvetica Chimica Acta, 1988, 71, 1191-1198.
- F. Seela and K. Kaiser, Helvetica Chimica Acta, 1988, 71, 1813-1823.
- F. Seela and G. Becher, Nucleic Acids Res., 2001, 29, 2069-2078.
- L.V. Nechev, I. Kozekov, C.M. Harris, and T.M. Harris, Chem Res Toxicol, 2001, 14, 1506-1512.
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