5,6-Dihydrothymidine, Dihydro-dT Oligonucleotide Modification
5,6-Dihydrouridine, Dihydro dU [dihydro-dU], is primarily used in studies of DNA damage and repair caused by oxidation, alkylation, free radicals, and by ultraviolet and ionizing radiation. In the cell, dihydrothymidine (DHU) DNA lesions are formed by gamma irradiation of deoxythymine under anoxic conditions. The body has evolved and adapted, generating a number of reparative enzyme systems to excise and repair these lesions resulting in the addition of hydrogen at C5 and C6 of the thymine ring. 5,6-Dihydrouridine are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and hydroxy pyrimidines are by products of major base damage formed by exposure of DNA to ionizing radiation.
Product Information
5,6-Dihydrothymidine, Dihydro-dT Oligonucleotide Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- L. Augeri, Y.M. Lee, A.B. Barton, and P.W. Doetsch, Biochemistry, 1997, 36, 721-729.
- P.A. Giannaris and M.J. Damha, Nucleic Acids Research, 1993, 21, 4742-4749.
- Shikazono, N., Pearson, C., O'Neill, P., Thacker, J. The roles of specific glycosylases in determining the mutagenic consequences of clustered DNA. Nucleic Acids Res. (2006), 34: 3722-3730. - dihydro dT (5-6 DHT)
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