2,6-Diaminopurine-2'-deoxyriboside (DAPdR) Oligonucleotide Modification
2,6-Diaminopurine-2'-deoxyriboside, 2-Amino-2'-deoxyadenosine, DAPdR are deoxyadenosine analogs. These modified DNA analogs are often used to substitute A bases to increase primer duplex stability since the 2-amino-A-T base pair is equivalent in strength to the G-T base pair. 2-Amino-A also destabilizes A-G wobble mismatches, thus increasing specificity.
See a list of our duplex stabilizing bases
Product Information
2,6-Diaminopurine-2'-deoxyriboside (DAPdR) Oligonucleotide Modification
Modified Bases; Duplex Stabilizing Base
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
1. "Metabolic activation of 2,6-diaminopurine and 2,6-diaminopurine-2′-deoxyriboside to antitumor agents" by GisbertWeckbeckerJoseph G.Cory; Advance in Enyzme Regulation Volume 28, 1989 p.125-144
2. Fernández-Sierra M, Shao Q, Fountain C, Finzi L, Dunlap D.E. coli Gyrase Fails to Negatively Supercoil Diaminopurine-Substituted DNA.J Mol Biol. 2015 Jul 3
3. Lazinski DW, Camilli A. Homopolymer tail-mediated ligation PCR: a streamlined and highly efficient method for DNA cloning and library construction. Biotechniques. 2013 Jan;54(1):25-34.
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