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Oligonucleotide Modification for Chemical Ligation

Bio-Synthesis offers several unnatural bases or functional group oligonucleotide modifications for use in chemical ligation applications. These modifications include: 5'-iodothymidine (5'-iodo group), 3'-thiophosphate, and various modifications for click ligation.

Modifications use for chemical ligation can be incorporated at any position within an oligonucleotide at time of synthesis or post-synthetically. Dual HPLC purification is highly recommended for post-synthetic conjugation.

Contact us for Oligonucleotide Modification for Chemical Ligation.

Our Advantages

  • Wide selection of modified RNA,DNA bases beyond 5-Mthyl C
  • Flexibility in oligo length and synthesis scales
  • Oligonucleotides are delivered as deprotected, desalted and/or purified
  • Quality checked by MALDI-TOF/LC-MS, analytical HPLC, PAGE
  • Specialize in producing newly-discovered and difficult-to-make modified bases
Name Modification Code* 5 Prime Internal 3 Prime Links
5' Iodothymidine [5IdT] X Details
5'-Hexynyl [5Hexnyl] X Details
3'-Thiophosphate [3SP] X Details
3'-Progargyl-5-Me-dC [X] X Details
5-Octadiynl dU [X] X Details
3Azide [Azide] X X X Details
Azido-PEG3 [X] X X X Details
Azido-PEG4 [X] X X X Details
Azido-PEG8 [X] X X X Details
Azido-PEG12 [X] X X X Details
Azido-PEG24 [X] X X X Details
Azido-Propylamine [X] X X X Details
5-Octadinyl dU [X] X X X Details
C8-Alkyne-dT (single Alkyne) [X] X X X Details
5'-C9-Alkyne [X] X Details
5'-C15-Alkyne [X] X Details
Alkyne-PEG3 [X] X X X Details
Alkyne-PEG4 [X] X X X Details
Alkyne-PEG8 [X] X X X Details
Alkyne-C6-dT [X] X X X Details
C8-TIPS-Alkyn-dT [X] X X X Details
C8-TMS-Alkyne-dT [X] X X X Details
C8-Alkyne-dC (5-Octadiynyl dC) [X] X X X Details
C8-TIPS-Alkyn-dC [X] X X X Details
C8-TMS-Alkyne-dC [X] X X X Details
C8-Alkyne dU (5-Octadiynyl dU) [X] X X X Details
5-Ethynyl-dU [X] X X X Details

* [x] = User define

Quality Control: Analytical HPLC, Gel eletrophoresis, and  MALDI-TOF Mass Spectrometry

Delivery times: 5-7 Working days

Packaging:    Dried

Shipping conditions: Room temperature

Storage conditions: -20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

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Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please Call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."