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Metal Chelate Oligo Modification

Bio-Synthesis offers metal chelate oligo modification services. These chelate-ligand modified oligonucleotides are suitable for in vivo siRNA imaging,1 or sequence selective DNA cleavage.2 Researchers have prepared metallo-DNA supramolecular assemblies that raise the fascinating possibility of metal-based molecular nano-devices such as molecular scale magnets and wires.3,4 Metallobases can be used to precisely control modified DNA through the addition (or removal) of a small amount of selected metal ion, which may lead to astonishing advancements in oligonucleotide research. Several groups have already reported exceptionally stable unnatural metallobase pairs using ligand modified oligonucleotides.5-8

Oligo metal-ligand labeling can be incororated into an oligonucleotide at the time of synthesis or conjugate post-synthetically via various type of cross-linking chemistry. Post-synthetic chemical modifications made to an oligonucleotide require dual HPLC purification. Contact Bio-Synthesis for metal chelate oligo modifications.

Bio-Synthesis metal chelate conjugates can be incorporate at any position of an oligonucleotide. Dual HPLC purification is required. Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

Metal Chalates Oligo Labelings Chemistry 5' Prime Internal 3' Prime
2,2'-Dipicolylamine Phosphoramidite Chemistry Y Y Y
DTPA Cross Linking Chemistry Y Y Y
DOTA Cross Linking Chemistry Y Y Y
NOTA Cross Linking Chemistry Y Y Y
TETA Cross Linking Chemistry Y Y Y
DTTA Cross Linking Chemistry Y Y Y

Quality Control: Analytical HPLC, Gel eletrophoresis, and  MALDI-TOF Mass Spectrometry

Delivery times: 5-7 Working days

Packaging:    Dried

Shipping conditions: Room temperature

Storage conditions: -20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

References :
  1. 1. Bartlett, D. W.; Su, H.; Hildebrandt, I. J.; Weber, W. A.; Davis, M. E., Impact of tumor-specific targeting on the biodistribution and efficacy of siRNA nanoparticles measured by multimodality in vivo imaging. Proc Natl Acad Sci U S A 2007, 104, (39), 15549-54.
  2. Dreyer, G. B.; Dervan, P. B., Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II). Proc Natl Acad Sci U S A 1985, 82, (4), 968-72.
  3. Tanaka, K.; Tengeiji, A.; Kato, T.; Toyama, N.; Shionoya, M., A discrete self-assembled metal array in artificial DNA. Science 2003, 299, (5610), 1212-3.
  4. Shionoya, M.; Tanaka, K., Artificial metallo-DNA: a bio-inspired approach to metal array programming. Curr Opin Chem Biol 2004, 8, (6), 592-7.
  5. Weizman, H.; Tor, Y., 2,2’-Bipyridine ligandoside: a novel building block for modifying DNA with intra-duplex metal complexes. J Am Chem Soc 2001, 123, (14), 3375-6.

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