Janelia Fluor Dye

Janelia Fluor® Dyes

Janelia Fluor^® Dyes are tetramethylrhodamine (TMR) based fluorescent dyes that are bright, photostable and cell-permeable. The dyes are suitable for confocal fluorescent imaging and super-resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). These dye series can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. DualHPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

	λabs	λem	Emission Color	Alternative
Janelia Fluor 525	525 nm	549 nm		Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532
Janelia Fluor 549	549 nm	571 nm		Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3
Janelia Fluor 585	585 nm	609 nm		Alexa Fluor® 568, Atto Rho12, Atto Rho101, CF594
Janelia Fluor 635	635 nm	652 nm		Alexa Fluor® 633, Atto 633, CF633
Janelia Fluor 646	646 nm	664 nm		Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5
Janelia Fluor 669	669 nm	682 nm		Alexa Fluor® 660, Atto 655, Atto 665, CF660R
Photoactivatable
PA Janelia Fluor 549	551-553 nm	570-573 nm		Activated at 365 nm.
PA Janelia Fluor 646	649-651 nm	663-665 nm		Activated at 365 nm.