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Intercalator Oligonucleotide Conjugates

The selective recongintion of nucleic acid base sequences is critical in molecular detection and therapeutic system. While gene expression is controled by sequence-specific regulatory proteins (repressor or activators) which shown to recognize the major groove of the DNA, however, many DNA-binding drugs often bind to the minor groove. Intercalating modified oligonucleotide are positively charged polycyclic aromatic molecules, which usually bind between two consecutive base pair of double-helical DNA via the minor groove. Thermodynamic studies have shown that intercalator modified oligonucleotide enhance binding specificity and stability due to the additional binding energy provided by the intercaltor. Short oligonucletoides can thereby reach a binding strength under physiological conditions that is sufficint to elicit a biological response. Intercalating modified oligonucleotides also made to resistant nuclease. These oligo modification often used extensively in the study of DNA structure and mutagenic, antiviral, antimitotic, anticarcinogenic,antimalaria activity

Contact Bio-Synthesis for triplex forming Intercalator modified oligonucleotide conjugates.

Bio-Synthesis intercalator bases can be incorporate at any position of an oligonucleotide . Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

Intercalator Base Modification Code 5' Prime Internal 3' Prime
Acridine [Acr] Y N Y
5-(1-Pyrenylethynyl)-2'-deoxyuridine [Pyrene-dU ] Y Y Y
5-(3-perylenylethynyl)-2'-deoxyuridine [Perylene-dU] Y Y Y
Psoralen C6 [Psoralen] Y Y Y

Quality Control: Analytical HPLC, Gel eletrophoresis, and  MALDI-TOF Mass Spectrometry

Delivery times: 5-7 Working days

Packaging:    Dried

Shipping conditions: Room temperature

Storage conditions: -20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

References :
  1. 1. Bartlett, D. W.; Su, H.; Hildebrandt, I. J.; Weber, W. A.; Davis, M. E., Impact of tumor-specific targeting on the biodistribution and efficacy of siRNA nanoparticles measured by multimodality in vivo imaging. Proc Natl Acad Sci U S A 2007, 104, (39), 15549-54.
  2. Dreyer, G. B.; Dervan, P. B., Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II). Proc Natl Acad Sci U S A 1985, 82, (4), 968-72.
  3. Tanaka, K.; Tengeiji, A.; Kato, T.; Toyama, N.; Shionoya, M., A discrete self-assembled metal array in artificial DNA. Science 2003, 299, (5610), 1212-3.
  4. Shionoya, M.; Tanaka, K., Artificial metallo-DNA: a bio-inspired approach to metal array programming. Curr Opin Chem Biol 2004, 8, (6), 592-7.
  5. Weizman, H.; Tor, Y., 2,2’-Bipyridine ligandoside: a novel building block for modifying DNA with intra-duplex metal complexes. J Am Chem Soc 2001, 123, (14), 3375-6.

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