Why the binding column capacity of BAC DNA MiniPrep is 25 ug, but recovery is only 0.6-1 ugs?
The reason for the difference between binding capacity and recovery is that the column can bind 25 ug but the typical yield from a 3 mL culture would be 0.6 to 1 ug in general due to the low copy number typical for BACs.
This kit is designed to process either:
• 50 x 1.5 mL culture samples
• 30 x 5 mL culture samples
• 15 x 10 mL culture samples
• 6 x 40 mL culture samples
• 3 x 100 mL culture samples.
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11/08/2012