lists many of the fluorescent dyes currently available with their excitation and emission wavelengths and their most optimal quencher option.
|
Dye-quencher compatibility
|
|
Dye Name
|
Ex.
|
Em.
|
BHQ Quenchers
|
Quenchers
|
|
Alexa 350
|
346
|
442
|
BHQ-0
l(MAX)=
495nm
Range=
430-520nm
|
QSY-35
l(MAX)=
475nm
|
|
DABCYL
l(MAX)=
479nm
|
|
Pacific Blue
|
416
|
451
|
|
Marina Blue
|
362
|
459
|
Eclipse
l(MAX)=
522nm
|
|
Acridine
|
362
|
462
|
|
Edans
|
336
|
468
|
|
Coumarin
|
432
|
472
|
|
BODIPY 493/503
|
493
|
503
|
|
Cy2
|
489
|
506
|
|
|
BODIPY FL-X
|
504
|
510
|
|
DANSYL
|
335
|
518
|
|
|
|
Alexa 488
|
495
|
519
|
BHQ-1
l(MAX)=
534nm
Range=
480-580nm
|
|
FAM
|
495
|
519
|
|
Oregon Green
|
500
|
520
|
|
Rhodamine Green-X
|
503
|
528
|
|
NBD-X
|
466
|
535
|
|
TET
|
521
|
536
|
|
Alexa 430
|
434
|
541
|
QSY-7&9
l(MAX)=
560nm
|
|
BODIPY R6G-X
|
529
|
547
|
|
JOE
|
520
|
548
|
|
Yakima Yellow
|
531
|
549
|
|
Alexa 532
|
532
|
554
|
|
VIC
|
538
|
554
|
|
HEX
|
535
|
556
|
|
R6G
|
524
|
557
|
BHQ-2
l(MAX)=
579nm
Range=
550-650nm
|
|
Alexa 555
|
555
|
565
|
|
BODIPY 564/570
|
563
|
569
|
|
BODIPY TMR-X
|
544
|
570
|
|
Cy3
|
550
|
570
|
|
Alexa 546
|
556
|
573
|
|
TAMRA
|
555
|
576
|
|
Rhodamine Red-X
|
560
|
580
|
|
BODIPY 581/591
|
581
|
591
|
|
Redmond Red
|
579
|
595
|
|
Cy3.5
|
581
|
596
|
|
|
ROX
|
575
|
602
|
|
Alexa 568
|
578
|
603
|
|
Cal Red
|
583
|
603
|
|
Texas Red
|
589
|
610
|
|
BODIPY TR-X
|
588
|
616
|
|
Alexa 594
|
590
|
617
|
|
BODIPY 630/650-X
|
625
|
640
|
BHQ-3
l(MAX)=
680nm
Range=
620-730nm
|
|
LC Red 640
|
625
|
640
|
|
Alexa 633
|
632
|
647
|
|
BODIPY 650/665-X
|
646
|
660
|
QSY-21
l(MAX)=
661nm
|
|
Alexa 647
|
650
|
665
|
|
Cy5
|
649
|
670
|
|
Alexa 660
|
663
|
690
|
BBQ650
l(MAX)=
650nm
Range=
550-750nm
|
|
Cy5.5
|
675
|
694
|
|
Alexa 680
|
679
|
702
|
|
Alexa 700
|
702
|
723
|
|
|
Alexa 750
|
749
|
775
|
See dye listings, quencher pair and emission color spectrum
Pdf file contain information on how to choose reporter-quencher pairs for efficient quenching through formation of intramolecular dimers.