Correctly designed antisense oligonucleotides (ASOs), siRNAs, and gapmers allow down-regulation or knocking out tRNAs and their fragments. However, the target sequence may need to be carefully selected.
Kanda et al. (1998) described how to knock out or knock down tRNAs by hybridizing antisense oligonucleotides. Please review this approach. However, modern gapmers also allow the recruitment of RNase H to initiate the degradation of the targeted sequences. Another option is the use of blocking ASOs to inhibit the action of the tRNA.
Since the antisense field has dramatically improved since this paper was published, designing more stable modified ASOs is now possible.
And yes, we have you covered. We offer a range of oligonucleotides for all three options.
Reference
Kanda T, Takai K, Yokoyama S, Takaku H. Knocking out a specific tRNA species within unfractionated Escherichia coli tRNA by using antisense (complementary) oligodeoxyribonucleotides. FEBS Lett. 1998 Dec 4;440(3):273-6.