What is PCR amplification

PCR amplification is the selective amplification of DNA or RNA targets using the polymerase chain reaction. During PCR, short single-stranded (ss) synthetic oligonucleotides or primers are extended on a target template using repeated cycles of heat denaturation, primer annealing, and primer extension.

The polymerase chain reaction or PCR has been one of the most game-changing or enabling technologies in the recent history of technical advances in molecular biology. PCR is an in vitro nucleic acid amplification method. PCR allows the amplification of DNA sequencing in an exponential way using repeated thermal cycling. PCR allows the generation of many millions of copies of DNA using heating and cooling cycles. As a result, many people are now using PCR in their research all over the world.