Peptide Nucleic Acid (PNA) oligonucleotides have a neutral peptide-like backbone, typically composed of repeating N-(2-aminoethyl)-glycine units and exhibit high binding affinities, resist enzymatic degradation, allow sequence-specific targeting. PNAs enable the design of antisense agents (ASOs) to block mRNA translation or as transcription factor decoys to inhibit gene expression.
PNAs will allow the design of fluorescence in situ hybridization (FISH) probes, microarray assays, and biosensors. Due to their high specificity and stability, PNAs can be used in triplex formation or as part of gene-editing strategies. Conjugation of PNAs to cell-penetrating peptides (CPPs), cholesterol, or lipids and chemical modification are also possible.
How to dissolve or reconstitute PNAs
Most PNA oligonucleotides dissolve well in pure or DI water. Occasionally, PNAs may not dissolve well in water based on sequences or modifications present.
In case of having technical difficulties in dissolving PNA oligomers, please follow the guidelines below:
Heat the aqueous solution to 60 °C for approximately 10 min.
Add 0.1% TFA or 10-20% acetonitrile or acetic acid to the aqueous solution.
If PNAs do not dissolve well in 0.1% TFA or 10 20% acetonitrile, test other organic solvents such as DMF, NMP, or similar.
Please note: Avoid using organic solvents if the PNA oligo is used for PCR.
Links
https://www.biosyn.com/PNAOverview.aspx
https://www.biosyn.com/tew/Adding-a-G-clamp-improves-the-thermodynamic-stability-of-the-DNA-duplex.aspx
https://www.biosyn.com/faq/What-is-BNA-PCR-clamping.aspx
https://www.biosyn.com/tew/bna-pna-lna-dna-comparison.aspx
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