I am planning an electroporation experiment in primary human T cells using mRNA that can efficiently be translated into a membrane-bound transporter of interest.
Question: For this particular experiment (mRNA transfection), are there any specific biochemical modifications that the mRNA should have?
Answer: No, no special modification is required for this application. However, incorporating 5-Methyl CTP and pseudo-UTP modifications into mRNA may reduce cellular immune-response to transfected mRNA.