Enzyme Modification and Conjugation
Various enzymes, such as HRP and AP, can be attached to antibodies and proteins to act as signal-generating molecules. Enzymes may be modified to contain homobifunctional or heterobifunctionalreactive groups that can covalently couple to some chemical target on the enzyme and result in a terminal reactive group that can crosslink with another molecule.
Enzymes can be covalently coupled to our clients’ specific antibody, oligonucleotide or peptides and other chemical compounds using:
- SMCC-Activated Enzymes
- Periodate oxidation methods
- Glutaraldehyde methods
- Hydrazide-activated enzymes
- SPDP heterobifunctionalcross linking chemistry
Through the use of different coupling chemistries, we can maintain the activity of the antibody by directing the position of the label so that it does not interfere with the antigen binding site. In addition, non-enzymatic fluorescent labels and biotin can be coupled to specific antibodies, proteins, and peptides as a means of signal generation.
As each conjugation is unique, our support team will work closely with each client to identify their objective and determine proper coupling chemistry. As we are the experts in enzyme conjugation techniques, BSIcan provide a properly conjugated product quickly and efficiently.
Type of Enzyme:
- Horseradish Peroxidase (HRP)
- Alkaline Phosphatase (AP)
- ß-Galactosidase(also called lactase)
- Glucose Oxidase
Service
- Oligonucleotide-enzyme conjugation
- Enzyme-antibody conjugation
- Enzyme-peptide conjugation and more...
Contact us for a quotation or to enlist our Research & Development team to help you find the solution you are searching for.