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Oligo Redox Modifications for Electrochemical Detection

Over the last decade enormous advancement has been made in the field of biosensors. Dense oligo gene chips arrays have been successfully applied for transcritional profiling. While labeled DNA probe has been used in many of DNA diagnostic applications, these fluorescent DNA probes require expensive reagents and instrumentation. Bio-Synthesis provides electrochemical nucleic acid biosenor probes using redox active labels. The detection is based on electrochemical response of a redox active label at electrode surface when hybridization occurs. These redox active "hybridization indicators" and have high affinity for either ssDNA or dsDNA to transduce hybridization. Advantage using electrochemical nucleic acid biosensors includes potential of miniaturization, fast, ease-to-use, affordable and compatible with microfabrication platform.

Redox labels can be be incorporate at 5', 3' or internal position of an oligonucleotide using phosphoramidite chemistry or conjugate post-synthetically. Contact Bio-Synthesis for electrochemical redox oligo labeling services..

All oligonucleotide synthesized at Bio-Synthesis is quality check by using either MALDI-TOF mass spectrometry, analytical HPLC,or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform custom formulation and quality control tailored to meet your specific requirements.

Electron Transfer Redox Labels Chemistry 5' Prime Internal 3' Prime
Ferrocene Phosphoramidite Chemistry Y Y Y
Methylene Blue Phosphoramidite Chemistry Y Y Y
ATTO MB2 Cross Linking Chemistry Y Y Y
Erythrosine B Cross Linking Chemistry Y Y Y
Antraquinone Cross Linking Chemistry Y Y Y

Quality Control: Analytical HPLC, Gel eletrophoresis, and  MALDI-TOF Mass Spectrometry

Delivery times: 5-7 Working days

Packaging:    Dried

Shipping conditions: Room temperature

Storage conditions: -20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

References :
  1. P. Piunno, U. Krull, R. Hudson, M. Damha, H. Cohen, Anal. Chem. 67 (1995) 2635.
  2. J. Wang, Chem. Eur. J. 5 (1999) 1681
  3. Y. Okahata, Y. Matsunobo, K. Ijiro, M. Mukae, A. Murkami, K. Makino, J. Am. Chem. Soc. 114 (1992) 8299
  4. S.R. Mikklesen, Electroanalysis 8 (1996) 15
  5. E. Palecek, M. Fojta, Anal. Chem. 73 (2001) 75A

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