2,4-Dinitrophenyl (DNP) Oligo Modification

2,4-dinitrophenyl (DNP)

Oligonucleotides labelled with DNP (2,4-dinitrophenyl) can be used in analytical tests based on anti-DNP antibodies. This DNP with triethylene glycol (TEG) spacer can be introduced anywhere in the sequence and on either terminus.

TEG-Dinitrophenol	Desalted Qty.	5'-Mod	Internal	3' -mod
250 nmol	15 OD	$265	NA	NA
1.0 µmol	35 OD	$350	NA	NA
Dinitrophenol	Desalted Qty.	5'-Mod	Internal	po3' -mod
250 nmol	15 OD	NA	$280	NA
1.0 µmol	35 OD	NA	$380	NA

Purification

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo.Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD₂₆₀ In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

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